Clone Assay:Article Title: Acidosis-induced activation of anion channel SLAH3 in the flooding-related stress response of Arabidopsis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Oocyte expression vector for N-terminal fusion to pHluorin This study pNB1u #pH21 Oocyte expression vector for N-terminal fusion to sBit This study pNB1u #52 Oocyte expression vector for N-terminal fusion to lBit This study pNB1u #54 Insect cell expression vector for C-terminal fusion to GFP, a Thrombin protease recognition sequence followed by a 3xFlag and an octahistidine sequence This study, Novagen pIEX5 Cos-7 expression vector for N-terminal fusion to mEOS This study pcDNA3.1mEOS CDS of AtSLAH1, AtSLAH2, AtSLAH3 and AtSLAC1 were cloned into pNB1u for electrophysiological analysis and into pNB1u #21 for localization studies This study, Geiger et al.,17 Cubero-Font et al.,18 and Maierhofer et al.19 N/A CDS of AtSLAH3 was cloned into pNBIu #pH21 for electrophysiological analysis and pH measurements This study N/A CDS of AtSLAH3 was cloned into pNBIu #52 and pNBIu #54 for interaction studies This study N/A CDS of AtSLAH2 and AtSLAH3 were cloned into pcDNA3.1mEOS for PALM stoichiometry analysis and electrophysiological measurements This study N/A CDS of AtSLAH3 was cloned into pIEX5 for protein purification This study N/A CDS of PttSLAH3, CqSLAH3 and DmSLAH3 were cloned into pNB1u for electrophysiological analysis This study, Cubero-Font et al.18 and Jaborsky et al.67 N/A CDS of AtCPK21delEF was cloned into pNB1u for electrophysiological analysis in X. leavis oocytes. .. This study; Geiger et al.17 N/A CDS of AtSLAH3 S127A was cloned into pNB1u for electrophysiological analysis This study, Liu et al.27 N/A CDS of AtSLAH3 T187D was cloned into pNB1u for electrophysiological analysis This study, Geiger et al.17 N/A CDS of ZmSUT1 was cloned into pNB1u for electrophysiological analysis This study, Carpaneto et al.28 N/A CDS of CD86 in vector IRES-puro2 Fricke et al.35 Addgene Cat#98284 CDS of CTLA4 in vector IRES-puro2 Fricke et al.35 Addgene Cat#98285 Software and algorithms NanoTemper Analysis version 2.1.2333 NanoTemper https://nanotempertech.com/monolith/ KaleidaGraph 4.5 Synergy Software https://www.synergy.com/ MUSCLE algorithm Edgar68 http://www.drive5.com/muscle/ Prime v4.7 Schrödinger https://www.schrodinger.com/products/prime PROPKA 3.0 Søndergaard et al.69 and Olsson et al.70 https://github.com/jensengroup/propka Desmond module v4.8 Schrödinger https://www.schrodinger.com/products/ desmond Patchmaster v2x90.5 Heka Instruments https://www.heka.com/downloads/ downloads_main.html#down_ patchmaster_next IgorPro v6.37 Wavemetrics https://www.wavemetrics.com/downloads/ current ImageJ (Fiji) v1.52p ImageJ https://fiji.sc/ OriginPro 2018 (64-bit) SR1 b9.5.1.195 (Academic) OriginLab https://www.originlab.com rapidSTORM 2 Wolter et al.71 https://github.com/stevewolter/rapidstorm ClampFit 10.7 Liu et al.72 https://de.moleculardevices.com/products/ axon-patch-clamp-system/acquisition-andanalysis-software/pclamp-software-suite#specs Excel 365 v2103 Microsoft https://www.microsoft.com/de-de/icrosoft- 365/excel Clustal Omega v1.2.2 Sievers et al.73 https://www.ebi.ac.uk/Tools/msa/clustalo/ (Continued on next page) Current Biology 31, 1–11.e1–e9, August 23, 2021 e2 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER VMD (visual molecular dynamics) v1.9.3 Humphrey et al.74 http://www.ks.uiuc.edu/Research/vmd/ Adobe Illustrator 2020 v24.2 Adobe https://www.adobe.com Ion Flux Monitor (custom-built Labview-based software) LABVIEW 2017 v17.0f2 (32-bit) Dindas et al.75 and Böhm et al.76 N/A CorelDRAW Graphics Suite 2017 v19.1.0.419 Corel Corporation https://www.coreldraw.com/en/ Leica Application Suite LAS AF 2.7.3.9723 Leica Microsystems https://www.leica-microsystems.com/ products/microscope-software/ Other Monolith NT.115 NanoTemper https://nanotempertech.com/monolith/ #monolith Imaging-PAM (MAXI version) Heinz Walz GmbH https://www.walz.com/products/chl_ p700/imaging-pam_ms/maxi_version.html Luminoskan Ascent Labsystems/ThermoScientific N/A Article
Plasmid Preparation:Article Title: Acidosis-induced activation of anion channel SLAH3 in the flooding-related stress response of Arabidopsis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Oocyte expression vector for N-terminal fusion to pHluorin This study pNB1u #pH21 Oocyte expression vector for N-terminal fusion to sBit This study pNB1u #52 Oocyte expression vector for N-terminal fusion to lBit This study pNB1u #54 Insect cell expression vector for C-terminal fusion to GFP, a Thrombin protease recognition sequence followed by a 3xFlag and an octahistidine sequence This study, Novagen pIEX5 Cos-7 expression vector for N-terminal fusion to mEOS This study pcDNA3.1mEOS CDS of AtSLAH1, AtSLAH2, AtSLAH3 and AtSLAC1 were cloned into pNB1u for electrophysiological analysis and into pNB1u #21 for localization studies This study, Geiger et al.,17 Cubero-Font et al.,18 and Maierhofer et al.19 N/A CDS of AtSLAH3 was cloned into pNBIu #pH21 for electrophysiological analysis and pH measurements This study N/A CDS of AtSLAH3 was cloned into pNBIu #52 and pNBIu #54 for interaction studies This study N/A CDS of AtSLAH2 and AtSLAH3 were cloned into pcDNA3.1mEOS for PALM stoichiometry analysis and electrophysiological measurements This study N/A CDS of AtSLAH3 was cloned into pIEX5 for protein purification This study N/A CDS of PttSLAH3, CqSLAH3 and DmSLAH3 were cloned into pNB1u for electrophysiological analysis This study, Cubero-Font et al.18 and Jaborsky et al.67 N/A CDS of AtCPK21delEF was cloned into pNB1u for electrophysiological analysis in X. leavis oocytes. .. This study; Geiger et al.17 N/A CDS of AtSLAH3 S127A was cloned into pNB1u for electrophysiological analysis This study, Liu et al.27 N/A CDS of AtSLAH3 T187D was cloned into pNB1u for electrophysiological analysis This study, Geiger et al.17 N/A CDS of ZmSUT1 was cloned into pNB1u for electrophysiological analysis This study, Carpaneto et al.28 N/A CDS of CD86 in vector IRES-puro2 Fricke et al.35 Addgene Cat#98284 CDS of CTLA4 in vector IRES-puro2 Fricke et al.35 Addgene Cat#98285 Software and algorithms NanoTemper Analysis version 2.1.2333 NanoTemper https://nanotempertech.com/monolith/ KaleidaGraph 4.5 Synergy Software https://www.synergy.com/ MUSCLE algorithm Edgar68 http://www.drive5.com/muscle/ Prime v4.7 Schrödinger https://www.schrodinger.com/products/prime PROPKA 3.0 Søndergaard et al.69 and Olsson et al.70 https://github.com/jensengroup/propka Desmond module v4.8 Schrödinger https://www.schrodinger.com/products/ desmond Patchmaster v2x90.5 Heka Instruments https://www.heka.com/downloads/ downloads_main.html#down_ patchmaster_next IgorPro v6.37 Wavemetrics https://www.wavemetrics.com/downloads/ current ImageJ (Fiji) v1.52p ImageJ https://fiji.sc/ OriginPro 2018 (64-bit) SR1 b9.5.1.195 (Academic) OriginLab https://www.originlab.com rapidSTORM 2 Wolter et al.71 https://github.com/stevewolter/rapidstorm ClampFit 10.7 Liu et al.72 https://de.moleculardevices.com/products/ axon-patch-clamp-system/acquisition-andanalysis-software/pclamp-software-suite#specs Excel 365 v2103 Microsoft https://www.microsoft.com/de-de/icrosoft- 365/excel Clustal Omega v1.2.2 Sievers et al.73 https://www.ebi.ac.uk/Tools/msa/clustalo/ (Continued on next page) Current Biology 31, 1–11.e1–e9, August 23, 2021 e2 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER VMD (visual molecular dynamics) v1.9.3 Humphrey et al.74 http://www.ks.uiuc.edu/Research/vmd/ Adobe Illustrator 2020 v24.2 Adobe https://www.adobe.com Ion Flux Monitor (custom-built Labview-based software) LABVIEW 2017 v17.0f2 (32-bit) Dindas et al.75 and Böhm et al.76 N/A CorelDRAW Graphics Suite 2017 v19.1.0.419 Corel Corporation https://www.coreldraw.com/en/ Leica Application Suite LAS AF 2.7.3.9723 Leica Microsystems https://www.leica-microsystems.com/ products/microscope-software/ Other Monolith NT.115 NanoTemper https://nanotempertech.com/monolith/ #monolith Imaging-PAM (MAXI version) Heinz Walz GmbH https://www.walz.com/products/chl_ p700/imaging-pam_ms/maxi_version.html Luminoskan Ascent Labsystems/ThermoScientific N/A Article
Software:Article Title: Acidosis-induced activation of anion channel SLAH3 in the flooding-related stress response of Arabidopsis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Oocyte expression vector for N-terminal fusion to pHluorin This study pNB1u #pH21 Oocyte expression vector for N-terminal fusion to sBit This study pNB1u #52 Oocyte expression vector for N-terminal fusion to lBit This study pNB1u #54 Insect cell expression vector for C-terminal fusion to GFP, a Thrombin protease recognition sequence followed by a 3xFlag and an octahistidine sequence This study, Novagen pIEX5 Cos-7 expression vector for N-terminal fusion to mEOS This study pcDNA3.1mEOS CDS of AtSLAH1, AtSLAH2, AtSLAH3 and AtSLAC1 were cloned into pNB1u for electrophysiological analysis and into pNB1u #21 for localization studies This study, Geiger et al.,17 Cubero-Font et al.,18 and Maierhofer et al.19 N/A CDS of AtSLAH3 was cloned into pNBIu #pH21 for electrophysiological analysis and pH measurements This study N/A CDS of AtSLAH3 was cloned into pNBIu #52 and pNBIu #54 for interaction studies This study N/A CDS of AtSLAH2 and AtSLAH3 were cloned into pcDNA3.1mEOS for PALM stoichiometry analysis and electrophysiological measurements This study N/A CDS of AtSLAH3 was cloned into pIEX5 for protein purification This study N/A CDS of PttSLAH3, CqSLAH3 and DmSLAH3 were cloned into pNB1u for electrophysiological analysis This study, Cubero-Font et al.18 and Jaborsky et al.67 N/A CDS of AtCPK21delEF was cloned into pNB1u for electrophysiological analysis in X. leavis oocytes. .. This study; Geiger et al.17 N/A CDS of AtSLAH3 S127A was cloned into pNB1u for electrophysiological analysis This study, Liu et al.27 N/A CDS of AtSLAH3 T187D was cloned into pNB1u for electrophysiological analysis This study, Geiger et al.17 N/A CDS of ZmSUT1 was cloned into pNB1u for electrophysiological analysis This study, Carpaneto et al.28 N/A CDS of CD86 in vector IRES-puro2 Fricke et al.35 Addgene Cat#98284 CDS of CTLA4 in vector IRES-puro2 Fricke et al.35 Addgene Cat#98285 Software and algorithms NanoTemper Analysis version 2.1.2333 NanoTemper https://nanotempertech.com/monolith/ KaleidaGraph 4.5 Synergy Software https://www.synergy.com/ MUSCLE algorithm Edgar68 http://www.drive5.com/muscle/ Prime v4.7 Schrödinger https://www.schrodinger.com/products/prime PROPKA 3.0 Søndergaard et al.69 and Olsson et al.70 https://github.com/jensengroup/propka Desmond module v4.8 Schrödinger https://www.schrodinger.com/products/ desmond Patchmaster v2x90.5 Heka Instruments https://www.heka.com/downloads/ downloads_main.html#down_ patchmaster_next IgorPro v6.37 Wavemetrics https://www.wavemetrics.com/downloads/ current ImageJ (Fiji) v1.52p ImageJ https://fiji.sc/ OriginPro 2018 (64-bit) SR1 b9.5.1.195 (Academic) OriginLab https://www.originlab.com rapidSTORM 2 Wolter et al.71 https://github.com/stevewolter/rapidstorm ClampFit 10.7 Liu et al.72 https://de.moleculardevices.com/products/ axon-patch-clamp-system/acquisition-andanalysis-software/pclamp-software-suite#specs Excel 365 v2103 Microsoft https://www.microsoft.com/de-de/icrosoft- 365/excel Clustal Omega v1.2.2 Sievers et al.73 https://www.ebi.ac.uk/Tools/msa/clustalo/ (Continued on next page) Current Biology 31, 1–11.e1–e9, August 23, 2021 e2 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER VMD (visual molecular dynamics) v1.9.3 Humphrey et al.74 http://www.ks.uiuc.edu/Research/vmd/ Adobe Illustrator 2020 v24.2 Adobe https://www.adobe.com Ion Flux Monitor (custom-built Labview-based software) LABVIEW 2017 v17.0f2 (32-bit) Dindas et al.75 and Böhm et al.76 N/A CorelDRAW Graphics Suite 2017 v19.1.0.419 Corel Corporation https://www.coreldraw.com/en/ Leica Application Suite LAS AF 2.7.3.9723 Leica Microsystems https://www.leica-microsystems.com/ products/microscope-software/ Other Monolith NT.115 NanoTemper https://nanotempertech.com/monolith/ #monolith Imaging-PAM (MAXI version) Heinz Walz GmbH https://www.walz.com/products/chl_ p700/imaging-pam_ms/maxi_version.html Luminoskan Ascent Labsystems/ThermoScientific N/A Article
|
Addgene inc is a verified supplier 